FIG. 4.

Repression of translation from the 210-nt 5′ UTR by the intraleader 33-nt ORF. (A) Sequence of the BCV leader and of the peptide generated from the G5→A mutant leader. (B) Repression of translation. Equal amounts of transcript from the p210N (lanes 2 and 5) and p210(intraleaderORF)N (lanes 3 and 6) were translated in wheat germ extract or in rabbit reticulocyte lysate as indicated, and the products were separated by SDS-PAGE. Dried gels were exposed for autoradiography. Lane 1 contains radiolabeled molecular weight marker proteins; lanes 4 and 7 are products of translation reactions in which no RNA was added.