TABLE 1.
5′ untranslated regions on BCV mRNAs
| mRNA species (major protein product) | 5′ UTR sequencea | 5′ UTR lengtha (nt) |
|---|---|---|
| 1 (polymerase) | 5′ (L) UAAACUUUAUAAAAACAUCCACUCCCUGUAUUCUAUGCUUGUGGGCGUAGAUUUUUCAUAGUGGUGUCUAUAUU- | |
| CAUUUCUGCUGUUAACAGCUUUCAGCCAGGGACGUGUUGUAUCCUAGGCAGUGGCCCACCCAUAGGUCACAAUG | 210 | |
| 2 (32 kDa) | 5′ (L) UAAACUUUAAGAAUG | 77 |
| 2-1 (HE) | 5′ (L) UAAACUCAGUGAAAAUG | 79 |
| 3 (S) | 5′ (L) UAAACAUG | 70 |
| 4 (4.8 kDa) | 5′ (L) UAAACCUGUAAUG | 75 |
| 5 (12.7 kDa) | 5′ (L) UAGACCUUAUAACUUUAAGCAUUAUUAAUUGCCAAAGUUUCUAAGGUCACGCCCUAGUAAUG | 124 |
| 5-1 (E) | 5′ (L) CAAACAUUAUGAUAAAUAUUUUGGAGUAAUAACUGGUUUUACAGCAUUCGCUAAUACUGUAGAGGAGGCUGUUA- | |
| ACAAACUGGUUUUCUUAGCUGUUGACUUUAUUACUUGGCGGAGACAGGAGUUAAAUG | 193 | |
| 6 (M) | 5′ (L) CAAACAUUAUG | 73 |
| 7 (N) | 5′ (L) UAAACUUUAAGGAUG | 77 |
a
Includes the common leader (L) of 65 nt: 5′GAUUGUGAGCGAUUUGCGUGCGUGCAUCCCGCUUCACUGAUCUCUUGUUAGAUCUUUUUAUAAUC. The canonical sequence motif (UC[U/C]AAAC) formed at leader-mRNA junctions and the nonconforming sequence UCUAGAC formed at the leader-mRNA junction for the 12.7 mRNA are underlined. The start and stop codons for an ORF potentially encoding an 8-amino-acid peptide within the 210-nt 5′ UTR of mRNA 1 are identified with bold type in an open box. The start codons thought to initiate translation of the named mRNA species are identified with open boxes. Data come from references 7 and 18.