Table 1.
Primer and probe sequences.
| Pathogens | Primers and Probe | Sequences (5′ to 3′) | Size (bp) | Gene |
|---|---|---|---|---|
| PEDV | Forward | TACTCTGCGTTCTTGTATGG | 92 | M |
| Reverse | CTAGCCCATGCATCAAAAAG | |||
| Probe | TAMRA-AATAGCCATCTTGACACCATACA-FAM | |||
| TGEV | Forward | GTGACAAGATTTTATGGAAC | 157 | N |
| Reverse | CTTCCTTTGAAGTCCAATAG | |||
| Probe | HEX-CTAGACACAGATGGAACACATTCA-BHQ1 | |||
| PDCoV | Forward | ACTCCCTCCTAATGATAC | 87 | N |
| Reverse | GCAACATGAGGTTTAATAG | |||
| Probe | Texas Red-CAGCAACCACTCGTGTTACTT-BHQ2 | |||
| PoRV-A | Forward | CGAACACATGTACTATAAGA | 99 | VP7 |
| Reverse | CAGCTGTTATGTCAAGTATA | |||
| Probe | Cy5-TTGGACCTCCTACCTGAATTAC-BHQ3 |