Table 3.

Sensitivity results of the multiplex real-time PCR.

Pathogens	Concentration	Total	Positive	Detection Rate	90% Detection Rate
PEDV	1000 copies/μL	15	15	100%	>90%
	100 copies/μL	15	15	100%	>90%
	10 copies/μL	15	9	60%	<90%
TGEV	1000 copies/μL	15	15	100%	>90%
	100 copies/μL	15	15	100%	>90%
	10 copies/μL	15	0	0%	<90%
PDCoV	1000 copies/μL	15	15	100%	>90%
	100 copies/μL	15	15	100%	>90%
	10 copies/μL	15	3	20%	<90%
PoRVA	1000 copies/μL	15	15	100%	>90%
	100 copies/μL	15	15	100%	>90%
	10 copies/μL	15	6	40%	<90%

Negative was determined by Cq ≥ 35 and positive was validated by Cq < 35.