Table 4.

Repeatability results (Cq value) of the multiplex real-time PCR.

Plasmid	Dilutions	Intra-Assay		Inter-Assay
		Mean ± SD	CV%	Mean ± SD	CV%
PoRVA	107	14.92 ± 0.05	0.37	15.00 ± 0.09	0.60
	106	17.35 ± 0.01	0.10	17.38 ± 0.04	0.25
	105	22.25 ± 0.13	0.59	20.27 ± 0.64	3.18
	104	24.63 ± 0.08	0.34	24.70 ± 0.07	0.28
	103	29.35 ± 0.34	1.17	29.31 ± 0.25	0.86
	102	32.51 ± 0.53	1.64	32.95 ± 0.35	1.08
	NTC	ND	ND	ND	ND
TGEV	107	15.27 ± 0.04	0.29	15.32 ± 0.07	0.46
	106	19.11 ± 0.08	0.40	19.04 ± 0.13	0.66
	105	21.11 ± 0.04	0.19	21.19 ± 0.05	0.23
	104	24.14 ± 0.03	0.13	24.16 ± 0.03	0.12
	103	27.58 ± 0.08	0.28	27.63 ± 0.06	0.21
	102	31.67 ± 0.15	0.47	31.55 ± 0.36	1.15
	NTC	ND	ND	ND	ND
PEDV	107	15.03 ± 0.06	0.40	15.04 ± 0.14	0.91
	106	18.36 ± 0.02	0.08	18.40 ± 0.02	0.11
	105	22.40 ± 0.11	0.50	23.34 ± 0.45	1.91
	104	26.57 ± 0.07	0.27	26.74 ± 0.25	0.92
	103	28.99 ± 0.25	0.87	28.88 ± 0.05	1.74
	102	32.42 ± 0.33	1.03	32.55 ± 0.46	1.37
	NTC	ND	ND	ND	ND
PDCoV	107	11.82 ± 0.15	1.27	12.44 ± 0.32	2.54
	106	15.68 ± 0.08	0.48	15.74 ± 0.13	0.85
	105	19.27 ± 0.08	0.42	19.13 ± 0.23	1.18
	104	22.54 ± 0.08	0.35	22.61 ± 0.26	1.14
	103	25.23 ± 0.12	0.46	25.44 ± 0.40	1.58
	102	28.02 ± 0.14	0.5	28.50 ± 0.47	1.65
	NTC	ND	ND	ND	ND