Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 26;14:17233. doi: 10.1038/s41598-024-68107-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Molecular dynamics simulations of Cas9 hybrids. (A) Domain organization of the Sp, Sa, and Cj Cas9 as well as the hybrids tested here. The PAM sequence is either the reported ones or, for the hybrids, the expected PAM. The numbers indicate the position of amino acids at domain boundaries (BH bridge helix, WED wedge domain, PID PAM interacting domain). (B) Schematic representation of the sgRNA hybrids tested here. (C) RMSD of SpCas9, SaCas9, and SaSpCas9 with their specific sgRNAs during 100 ns. The SaSp sgRNA presented is Merry. (D) RMSD of SpCas9, CjCas9, and CjSpCas9 with their specific sgRNAs over a simulation of 100 ns. The CjSp sgRNA presented is Bilbo. (E–G) Average RMSD for the protein (E), sgRNA (F), and DNA (G) in all 12 systems modeled. (H) RMSF of SaCas9 with its sgRNA (black) and SaSpCas9 in combination with Merry (red) for the N-terminal common to both proteins. (I) RMSF of the PID of SpCas9 with its sgRNA (yellow) and SaSpCas9 in combination with Merry (red). (J) RMSF of SpCas9 sgRNA (yellow), SaCas9 sgRNA (black) and SaSpCas9 sgRNA (red).