FIG. 3.

Coexpression of C-prM mutants and NS2B-3₁₈₁. Expression of the transfected plasmids was driven by a vaccinia virus recombinant that expresses T7 RNA polymerase (vTF7-3). The C-prM cassettes contained the mutations at the dibasic capsid site that are listed in Table 1. SDS lysates were immunoprecipitated with antiserum to either prM (A) or C (B). Mock infection, vTF7-3 alone, and pTM3-NS2B-3₁₈₁ without pTM3-C-prM are shown in the first three lanes. Wild-type (WT) and mutant C-prM are indicated, either without (−) or with (+) cotransfection of pTM3-NS2B-3₁₈₁. Positions of molecular mass standards ([¹⁴C]MW markers) are shown, with sizes in kilodaltons noted on the left (the 12.5- and 14.3-kDa markers comigrate on SDS–13% PAGE). The positions of C, prM, and the C-prM polyprotein are indicated on the right.