FIG. 4.

Coexpression of wild-type C-prM and NS2B-3₁₈₁ components. Following infection with vTF7-3, BHK-21 cells were transfected with pTM3-C-prM alone (lane 3) or in combination with pTM3-NS2B (lane 4), pTM3-NS3₁₈₁ (lane 5), pTM3-NS3₁₈₁(S→A) (lane 6), pTM3-NS2B-3₁₈₁ (lane 7), pTM3-NS2B plus pTM3-NS3₁₈₁ (lane 8), or pTM3-NS2B plus pTM3-NS3₁₈₁(S→A) (lane 9). Following immunoprecipitation with antiserum to prM (A) or a combination of antisera to NS2B and NS3 (B), proteins were separated by SDS–13% PAGE. Mock-infected cell lysate was immunoprecipitated in lane 1, and untransfected vTF7-3-infected cell lysate was immunoprecipitated in lane 2. The identities of the proteins are noted on the right side of each panel, and the positions of standards (in kilodaltons) are indicated on the left. A small amount of unprocessed NS2B-3₁₈₁ can be seen in lane 7 of panel B. NS3₁₈₁ has only a single internal methionine residue, while NS2B has four, contributing to the difference in band intensity.