FIG. 8.

Analysis of envelope protein distribution in cells transfected with full-length mutant transcripts of YF cDNA. (A) Transfected BHK-21 cells were labeled with [³⁵S]methionine for 6 h (18 to 24 h postelectroporation); media was harvested after the labeling period, solubilized with SDS, and immunoprecipitated with antiserum to the E protein. (B) SDS lysates of the cell monolayers from the above experiment were prepared and immunoprecipitated with E antiserum. (C) SDS lysates of the cell monolayers from the above experiment were prepared and immunoprecipitated with NS3 antiserum. Immunoprecipitation of medium or of lysate of cells transfected with no RNA is shown in the first lane of each panel. The positions of E and NS3 are indicated on the right side of their respective panels, and the positions of protein standards (in kilodaltons) are shown on the left. WT, wild type.