FIGURE 1.

Human T-cell expansion timelime. T-cells were stimulated on day 0 using ImmunoCult™ Human CD3/CD28/CD2 T-cell Activator in ImmunoCult™-XF T-cell Expansion Medium supplemented with IL-2 in static culture. Total culture volumes were increased by 8-fold using fresh medium on day 3. Total culture volumes were further increased by 4-fold on day 5 and transferred to the respective bioreactor systems. Basal media was added to the cultures by 4-folds on day 7 and day 10 until harvest on day 12 of the process.