FIG. 2.

In vitro translation of WSN NA cysteine-to-glycine mutants. Wild-type (WT) WSN NA and the indicated mutant WSN NAs were transcribed and translated in vitro in the presence of [³⁵S]methionine. (−), no-DNA negative control reaction. The products were run on a sodium dodecyl sulfate–10% polyacrylamide gel, which was then exposed to film. The arrowheads indicate positions of full-length, unmodified NA.