TABLE 2.
Relative activities of mutant NAs in the vaccinia virus-T7 system
| Mutation | Codon change(s) | % Activitya at:
|
||
|---|---|---|---|---|
| 33°C | 37°C | 39.5°C | ||
| C49G | TGC→GGT | 130 | 130 | 124 |
| C76G | TGT→GGG | <0.1 | <0.1 | <0.1 |
| C109G | TGT→GGA | <0.1 | <0.1 | <0.1 |
| C146G | TGT→GGG | 27.6 | 30.4 | 17.4 |
| C169G | TGT→GGA | <0.1 | <0.1 | <0.1 |
| C218G | TGT→GGA | <0.1 | <0.1 | <0.1 |
| C264G | TGT→GGA | <0.1 | <0.1 | <0.1 |
| C266G | TGT→GGA | <0.1 | <0.1 | <0.1 |
| C303G | TGC→GGA | 2.3 | 2.7 | 0.3 |
| C320G | TGT→GGG | 2.4 | 1.0 | 0.2 |
| C406G | TGC→GGA | <0.1 | <0.1 | <0.1 |
| C49G,C146G | TGT→GGA, TGT→GGG | 21.6 | 16.7 | 10.2 |
| C303G,C320G | TGC→GGA, TGT→GGG | <0.1 | <0.1 | <0.1 |
a
100% activity corresponds to the activity of wild-type WSN NA transfected in parallel at the indicated temperature. The activities of wild-type WSN NA were 3.64 ± 0.72 at 33°C, 4.41 ± 0.44 at 37°C, and 1.06 ± 0.14 pmol of Mu-NANA cleaved/s at 39.5°C.