TABLE 2.
Sequence analysis of donor-target junction sites produced by purified SIV INa
| Characteristic | Size (bp) | No. of recombinants
|
% of total recombinants | |||
|---|---|---|---|---|---|---|
| U5-U3
|
U5-U5
|
Total | ||||
| Donor with target; IN added | Donor with target; IN added | Donor complex to IN; target added | ||||
| Duplication | 5 | 49 | 35 | 34 | 118 | 79.2 |
| 6 | 2 | 1 | 0 | 3 | 2.1 | |
| 4 | 2 | 0 | 1 | 3 | 2.1 | |
| 0 | 6 | 2 | 1 | 9 | 6.0 | |
| Deletion | 17, 18 | 0 | 1 | 2 | 3 | 2.0 |
| 27–29 | 5 | 3 | 1 | 9 | 6.0 | |
| Larger | 3 | 0 | 1 | 4 | 2.7 | |
| Total | 67 | 42 | 40 | 149 | ||
a
The donor-target junctions of 149 recombinants produced from three independent reactions were sequenced. There were 11 additional clones selected that contained either only one or no LTR termini; some were smaller or had other aberrations suggesting rearrangements or deletions had occurred.