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. 2024 Jul 25;25:287. doi: 10.1186/s12931-024-02869-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Effects of HIF-1α on ASMCs’ biological functions. Note (A) ELISA detection of inflammation factor levels in ASMCs before and after modeling; (B) ELISA detection of inflammation factor levels in cells from each group; (C) MTT assay to measure cell viability in each group, “%control” refers to the cell viability of each group compared to the Normoxia group; (D) Flow cytometry analysis of cell cycle changes in each group; (E) EdU experiment to evaluate cell proliferation capacity in each group (scale bar: 20 μm); (F) Transwell assay to assess cell migration ability in each group; (G) Quantification of Transwell assay results; (H) TUNEL assay to measure cell apoptosis rate in each group (scale bar = 50 μm); (I) Quantification of TUNEL assay results. Statistical significance was denoted as follows: * for P < 0.05 compared to the Hypoxia + sh-NC group, ** for P < 0.01 compared to the Hypoxia + sh-NC group, $ for P < 0.05 compared to the Normoxia group, and $$ for P < 0.01 compared to the Normoxia group. Each cell experiment was replicated three times