Fig. 5.

Effects of the HIF-1α/MDM2/P53 axis on ASMCs’ biological functions. Note (A) Western blot detection of HIF-1α, MDM2, and P53 protein expression levels in cells from each group; (B) ELISA detection of inflammation factor levels in cells from each group; (C) MTT assay to measure cell viability in each group, “%control” refers to the cell viability of each group compared to the Normoxia group; (D) Flow cytometry analysis of cell cycle changes in each group; (E) EdU experiment to evaluate cell proliferation capacity in each group (scale bar: 20 μm); (F) Quantification of EdU experiment results; (G) Transwell assay to assess cell migration ability in each group; (H) TUNEL assay to measure cell apoptosis rate in each group (scale bar = 50 μm). Statistical significance was indicated by (*) when comparing with the Hypoxia + sh-NC + oe-NC group where P < 0.05, (**) when compared with the Hypoxia + sh-NC + oe-NC group where P < 0.01, (#) when compared with the Hypoxia + sh-HIF-1α + oe-NC group where P < 0.05, and (##) when compared with the Hypoxia + sh-HIF-1α + oe-NC group where P < 0.01. The cell experiments were repeated three times for reliability