Table 1.
Components of the Fixative
| Fixative | Components | Amount |
|---|---|---|
| Bouin’s solution | Picric acid saturated aqueous solution | 750 mL |
| 40% formaldehyde | 250 mL | |
| Glacial acetic acid | 50 mL | |
| Karnovsky fixative | Paraformaldehyde, 25% freshly prepared | 10 mL |
| Glutaraldehyde 50% | 10 mL | |
| Na-phosphate buffer (0.1 M, pH 7.4) | 50 mL | |
| Distilled water | 30 mL | |
| Na-phosphate buffer (0.1 M, pH 7.4) | Solution A | |
| Na2HPO4·2H2O | 17.02 g | |
| Distilled water | 600 mL | |
| Solution B | ||
| NaH2PO4·H2 | 6 g | |
| Distilled water | 200 mL | |
| Using solution | ||
| Solution A | 580 mL | |
| Solution B | 219 mL | |
| Citrate buffer (pH 6.0) | Solution A | |
| Citrate C6H8O7·H2O | 21 g | |
| Distilled water | 1 L | |
| Solution B | ||
| Sodium citrate Na3C6H5O7·2H2O | 29.41 g | |
| Distilled water | 1 L | |
| Using solution | ||
| Solution A | 9 mL | |
| Solution B | 41 mL | |
| Distilled water | Add 500 mL | |