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. 2024 Jul 27;43:209. doi: 10.1186/s13046-024-03129-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — IL-33 stimulates the release of EV by eosinophils. EV were isolated from Eo5 and Eo33 conditioned media by serial ultracentrifugations. A NTA analysis of vesicle size and (B) transmission electron microscopy of negative stained Eo5-EV and Eo33-EV. Western blotting for (C) EV markers Alix and Tsg101 in Eo5-EV and Eo33-EV and (D) GM130 and Calnexin in Eo5-EV, Eo33-EV and their producing cells. Flow cytometry quantification of fluorescent EV released by Bodipy FL-C16 labelled Eo5 and Eo33 (E) cultured alone or (F) in the presence of B16.F10 melanoma cells (20:1 ratio) for 24 h. Data are expressed as number of EV released per cell. Mean (SD) of four (E) and three (F) separate experiments is shown. G-H Transmission electron microscopy analysis of Eo33 co-cultured with B16.F10 melanoma cells (10:1 ratio) for 1 h, showing the presence of multivesicular bodies (MVB, G) and EV release (H) by an eosinophil in proximity of a tumor cell. Gr: granule. Nu: nucleus