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. 2024 Jul 27;43:209. doi: 10.1186/s13046-024-03129-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Transfer of eosinophil-derived EV to tumor cells. A Eo5 and Eo33 were labelled with Bodipy FL-C16 and then co-cultured with tumor cells separated by a 0.4 μm pore sized insert. C16⁺ EV intake by tumor cells after the indicated culture times, revealed by flow cytometry analysis of green fluorescence acquisition. CTR: tumor cells cultured alone. Mean (SD) of three replicates is shown. (B) On chip assay for visualizing eosinophil-derived EV incorporation by B16.F10 tumor cells by fluorescence microscopy. C16-labelled Eo5 or Eo33 and B16.F10 cells were loaded in the chip as indicated and incubated for 18 h. Images depict C16⁺ fluorescence (green) in B16.F10 cells at the beginning of the culture (T = 0 h) and after 18 h incubation. Inserts represent separate visible and green fluorescence channels. Scalebar: 100 μm. C Microphotographs showing incorporation of C16⁺ EV (green) from Eo5 or Eo33 in the cytoplasm of B16.F10 cells after 18 h. Chips were fixed and stained with DAPI (blue) at the end of the co-culture. Scalebar: 5 μm