FIG. 5.

Analysis of gD binding to HveC in real time. HveC(346t) (A, C, and E) or HveC(143t) (B, D, and F) were immobilized on a CM5 biosensor chip to 1,600 and 300 RU, respectively, in a Biacore X instrument. Various concentrations of gD(306t) (A and B), gD(285t) (C and D), and gD(rid1t) (E and F) were flowed over the chip for 2 min (association) and then replaced by buffer for another 2 min (dissociation). Sensorgrams of corrected data are represented after the subtraction of signal from the control flow cell. Data points were collected at 5 Hz but, for clarity, only one every 25 points is represented here by a symbol. The solid line shows the best fit obtained after global fitting with the BIAevaluation 3.0 software (5).