FIG. 7.
Binding of soluble HveC to HSV particles. Purified HveC(346t) or HveC(143t) (100 μg) were incubated with 107 PFU of purified HSV-1 KOS for 90 min at 4°C. Viral particles were then sedimented through a discontinuous sucrose gradient. The virus-containing fraction was collected, concentrated, and analyzed by Western blot for detection of HveC and virus. Receptors were detected with anti-tetra-His antibody, and VP5 capsid protein was detected with NC-1 polyclonal rabbit serum simultaneously.