FIG. 9.

Quantitation of gD(285t) and HveC(143t) in the complex. Two aliquots of a fraction containing the gD(285t)-HveC(143t) complex separated by gel filtration (Fig. 8F) were loaded onto a 16% polyacrylamide gel (lanes 5 and 6). Standards of known amounts of HveC(143t) and gD(285t) were loaded in lanes 1 to 4 and lanes 7 to 10, respectively, with amounts of proteins as indicated under the gel. After the silver staining of the gel, the intensity of the protein bands was measured by densitometry. The amount of each protein in the complex is indicated and is based on the comparison with the standards.