FIG. 2.

Expression and release of Gag when coexpressed with wt Env or Env164RS. Cells expressing the Gag proteins were radiolabeled, and aliquots of cell lysate or medium were immunoprecipitated by SIV antiserum. For the detection of Gag from cells coexpressed with wt Env or 164RS, an aliquot from the samples of panels B and D in Fig. 1 was subjected to SDS–8% PAGE. The cells were labeled in a 30-min pulse and chased for 3, 5, 8, and 12 h, as indicated over the gels. Intracellular levels of Gag protein alone (A), Gag protein when expressed with wt Env protein (B), and Gag protein when expressed with Env164RS (C) and the Gag proteins released into the media after the chase periods (in hours) indicated over the gels.