FIG. 2.

PCR and Southern blot analysis specific for BLV SGV provirus. (Left) Reverse transcription converts the 3′ SNV LTR in vector DNA to a 3′ hybrid SNV-BLV LTR in BLV SGV provirus. The hybrid LTR is amplified by PCR with primers specific for BLV polypurine tract (PPT), which functions in the reverse transcription step of replication (forward primer KB504mod), and the U5 region of the BLV LTR (reverse primer KB572). Southern blot hybridization analysis with a ³²P-labeled SNV LTR probe is expected to detect a 650-bp product. (Right) Detection of 650-bp 3′ hybrid LTR PCR product by Southern blot analysis with a ³²P-labeled SNV LTR probe. Lanes are labeled with the sources of DNA. Lanes 3 and 4 contain 100 ng of cells. Lane 5, pGem DNA size standard (Promega).