Fig. 3. Functional analysis of spike-binding mAbs and their lineage tracing in bulk IgG repertoires.

a Summary of the number of spike-binding lineages and cloned spike-binding, RBD-binding and neutralizing mAbs isolated from H03. b ELISA EC₅₀ values based on binding to the ancestral prefusion D614G spike protein with neutralizing mAbs highlighted in red and non-neutralizing mAbs in white. Binding assays were performed in triplicates for each mAb. Source data are provided as a Source Data file. c Dissemination of spike-binding mAb lineages where the bubble size is directly proportional to the number of sequences identified for each lineage in each library. The x-axis of each section shows the sample compartment, grouped by time point with the sampling week indicated. Source data are provided as a Source Data file. d Summary table displaying V, D and J allele assignments, V gene SHM levels, CDR3 amino acid identity and IC₅₀ neutralizing titers against the D614G variant for each neutralizing mAb. Neutralization assays were performed in triplicates for each mAb. The table displays only one of the most likely possible D allele assignments.