Fig. 1. Overview of the bacteriocin expression platform.

A A high-copy plasmid codes for constitutive expression of a specific bacteriocin fused with one of four different secretion signals. The expressed bacteriocins are secreted from the host cells and can target susceptible species in the extracellular environment. The secretion signals used in this study have previously been reported to exit the cell via the SecYEG inner-membrane and the GspD outer-membrane pore. B The amino acid sequences of the EntA and EntB bacteriocins used in this study.