Fig. 3. Liquid co-culture characterisation of the individual bacteriocin expressing strains.

A The experimental protocol used for co-culture growth assays. B Growth curves of the competitor strains in co-culture. The labels indicate the bacteriocin construct produced by the engineered competitor strain. Lines indicate mean and points show individual measurements (n = 4 biological repeats). C The estimated ratio of E. faecalis OD over total OD at 10 hours, when grown in co-culture with the indicated strain. Bars indicate mean values ± SE (n = 4 biological repeats). D Comparison of the solid culture inhibition zone vs the 10 hour ratio for each of the co-culture conditions. Solid line shows linear regression fit, with the R² value labelled. Points give mean value ± SE (n = 4 biological repeats). Source data are provided as a source data file.