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. 2024 Jul 27;14:17318. doi: 10.1038/s41598-024-67557-x

Figure 2.

Figure 2

ICA alleviates cisplatin-induced oxidative stress. (A) Representative IHC images of 4-HNE, NTY and 8-OHdG in ovaries of each group (n = 6). Scale bars, 100 µm. (B-C), Quantification of positive area for 4-HNE (B), NTY (C) and 8-OHdG (D). (E), MDA levels in each group’s ovarian tissue were determined (n = 4). (F), Representative IHC images of Nrf2, HO-1, and Gpx4 in ovaries of each group (n = 6). Scale bars, 100 µm. (G), Nrf2, HO-1 and Gpx4 protein expression levels in ovarian tissues of each group were examined by WB (n ≥ 3). (H-J), Quantification of Nrf2 (H), HO-1 (I), and Gpx4 (J) protein levels. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, non-statistically significant.