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. 2024 Jul 27;14:17318. doi: 10.1038/s41598-024-67557-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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ICA rescued cis-induced apoptosis in KGN cells. (A,B) Determination of γH2AX and Bax protein expression levels in KGN cells from different treatment groups by WB (A) and quantitative analysis (B,C). (D,E), Effect of ICA on the percentage of Cis-induced apoptosis in KGN cells using Annexin V/PI staining and flow cytometry. (F,G), Effect of ICA and Fer-1 on the percentage of RSL3-induced apoptosis in KGN cells using Annexin V/PI staining and flow cytometry. Data are presented as mean ± SEM, **P < 0.01, ****P < 0.0001, ns, non-statistically significant.