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. 2024 Jul 27;14:17318. doi: 10.1038/s41598-024-67557-x

Figure 8.

Figure 8

The Nrf2 inhibitor ML385 attenuates the protective effect of ICA on cis-induced KGN cell injury. (A–C) Effect of co-treatment of ICA with ML385 (2.5 μM) on cis-induced KGN cell viability (A), cytotoxicity (B) and MDA levels (C). (D) and (E), ROS were detected using DCFH-DA (D) and quantified (E). (F) and (G), Lipid peroxidation levels were detected using C11-BODIPY 581/591 probes (F) and quantified (G). (H) and (I), The percentage of apoptosis of KGN cells in different treatment groups was detected using Annexin V/PI staining and flow cytometry. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, non-statistically significant.