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. 1999 Oct;73(10):8185–8195. doi: 10.1128/jvi.73.10.8185-8195.1999

FIG. 2.

FIG. 2

Melting analyses of Mo-MuLV dimeric RNAs isolated from wild-type and CCHH and PR− mutant virions. RNAs extracted from the respective virions were resuspended and heated for 10 min at the temperatures indicated in the figure. The positions of the monomer and dimer RNA species are indicated at the left. A comparison of the melting temperatures of the wild type (WT), the CCHH mutant, and the PR− mutant RNA dimers is presented. Wild-type virus contains a “mature” RNA dimer, and PR− virus contains an “immature” RNA dimer (15). The melting analysis of the CCHH mutant RNA was compared with that of the wild-type RNA and the PR− RNA in separate experiments, but in the interest of saving space, only one CCHH analysis is presented. The melting analyses were identical for the CCHH mutant in both experiments.