FIG. 3.

PBS occupancy determination in genomic RNAs isolated from wild-type (WT) and CCHH mutant virions. (A) Primer tagging was performed to determine the levels of primer tRNA at the PBS. Undiluted and 1:10-diluted vRNAs were examined as indicated. Heated (samples heated to 100°C for 5 min and immediately chilled on ice prior to primer tagging) and unheated samples were tested, as indicated. (B) Nondenaturing Northern blot analysis of CCHH mutant and wild-type vRNA were tested undiluted and at a 1:10 dilution (RNA from 7.5 and 0.75 ml of culture supernatant, respectively) is indicated.