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TABLE 1.

Occupancy of the PBS in Mo-MuLV CCHH mutant and wild-type vRNA

Sample Genomic RNA content (% of wild type)a tRNA content of PBS (% of wild type)b tRNA/genomic RNA (% of wild type)c
Wild type undiluted 100 100 100
Wild type 1:10 Dilution 12.7 11.6 91
CCHH undiluted 30.8 41.4 134
CCHH 1:10 Dilution 1.3 5.2 400
a

vRNA band intensity from nondenaturing Northern blot analysis (Fig. 3B), measured by phosphorimaging. 

b

[α-32P]dAMP-radiolabeled tRNA band intensity from primer tagging (Fig. 3A), measured by phosphorimaging. 

c

Ratio of tRNA intensity to genomic RNA intensity expressed as a percentage of the wild-type value.