TABLE 1.
Sample | Genomic RNA content (% of wild type)a | tRNA content of PBS (% of wild type)b | tRNA/genomic RNA (% of wild type)c |
---|---|---|---|
Wild type undiluted | 100 | 100 | 100 |
Wild type 1:10 Dilution | 12.7 | 11.6 | 91 |
CCHH undiluted | 30.8 | 41.4 | 134 |
CCHH 1:10 Dilution | 1.3 | 5.2 | 400 |
vRNA band intensity from nondenaturing Northern blot analysis (Fig. 3B), measured by phosphorimaging.
[α-32P]dAMP-radiolabeled tRNA band intensity from primer tagging (Fig. 3A), measured by phosphorimaging.
Ratio of tRNA intensity to genomic RNA intensity expressed as a percentage of the wild-type value.