Table-1.
The effect of thermal manipulation at 12–18 days of incubation on some hematological and blood biochemical traits of roosters exposed to thermal challenge.
| Traits | G1 (Control) | G2 (TM) | p-value |
|---|---|---|---|
| WBCs (×103/mm3) | 21.46b ± 0.35 | 23.81a ± 0.92 | 0.018 |
| Monocyte (%) | 6.86 ± 0.29 | 7.45 ± 0.26 | 0.079 |
| Eosinophil (%) | 9.96 ± 0.18 | 9.77 ± 0.18 | 0.444 |
| Lymphocyte (%) | 40.96 ± 0.96 | 42.61 ± 1.05 | 0.312 |
| Heterophil (%) | 25.72a ± 0.91 | 21.20b ± 0.94 | 0.003 |
| H/L ratio | 0.63a ± 0.39 | 0.50b ± 0.32 | 0.021 |
| Total protein (g/dL) | 5.14b ± 0.16 | 6.24a ± 0.13 | 0.001 |
| Albumin (g/dL) | 3.65 ± 0.05 | 3.78 ± 0.05 | 0.068 |
| Globulin (g/dL) | 1.51b ± 0.13 | 2.48a ± 0.13 | 0.022 |
| IgG (mg/dL) | 3.62b ± 0.13 | 4.18a ± 0.16 | 0.014 |
| IgM (mg/dL) | 0.89b ± 0.01 | 1.25a ± 0.04 | 0.012 |
| IgA (mg/dL) | 0.02b ± 0.01 | 0.61a ± 0.11 | 0.000 |
| AST (U/L) | 58.23a ± 1.89 | 41.79b ± 0.40 | 0.000 |
| ALT (U/L) | 19.91a ± 0.38 | 16.83b ± 0.06 | 0.001 |
| TAC (mMol/dL) | 385.94b ± 5.92 | 441.37a ± 4.93 | 0.000 |
| Malondialdehyde (nmol/mL) | 1.17a ± 0.06 | 0.88b ± 0.03 | 0.003 |
| T3 (ng/mL) | 2.83b ± 0.07 | 3.88a ± 0.09 | 0.001 |
| T4 (ng/mL) | 14.76b ± 0.52 | 17.87a ± 0.15 | 0.001 |
| Corticosterone (ng/mL) | 25.31a ± 1.24 | 18.85b ± 1.03 | 0.003 |
| Testosterone (ng/dL) | 427.92b ± 3.31 | 446.86a ± 1.36 | 0.001 |
a,bMeans having different letters in the same row are significantly different (p ≤ 0.05). TM=Temperature manipulation, H/L ratio=Heterophils/lymphocytes ratio. n = 60 blood samples from each group, WBCs=White blood cell, IgG=Immunoglobulin G, IgM=Immunoglobulin M, AST=Aspartate aminotransferase, ALT=Alanine aminotransferase, TAC=Total antioxidant capacity