FIG. 5.

Induction of p53 and p53-responsive genes gadd45, p21, and mdm-2 by d106. Western blot analysis of the indicated proteins was performed at multiple times (hours postinfection [h.p.i.]) of HEL cells with d106 or d109 to assess the steady-state levels of candidate gene products identified on the ATLAS filters. Equal amounts of total cellular protein were separated by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and blotted with the indicated antibodies. Panels: A Western blot of cellular p53; B, Western blot of p21 and p53 of mock-infected (lanes M) versus d106-infected HEL cells; C, induction of mdm-2 and gadd45 as a consequence of ICP0 expression. Lanes 0 contained extracts from cells just prior to infection.