FIG. 5.

Characterization of env stable transfectants. (A) Tat-dependent HIV-1 env gene expression in stable env transfectants. The four env transfectants were transfected with or without 5 μg of pIIIextat. Equal amount of cell lysates from each transfection were subjected to an SDS–7.5% PAGE, followed by Western blotting by using MAbs Chessie 8 and Chessie 13. m.w., molecular size. (B) Analysis of Tat-induced Env expression in env transfectants by indirect immunofluorescence microscopy. The four env transfectants as indicated were transfected with pIIIextat. Three days after transfection, cells were processed for immunofluorescence analysis as described in Materials and Methods. Cells were analyzed under a differential interference contrast microscope (panels a, c, e, and g) or fluorescence microscope (panels b, d, f, and h), both at a magnification of 200×.