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. 2024 Jun 14;65(7):100579. doi: 10.1016/j.jlr.2024.100579

Fig. 2.

Fig. 2

Transcriptional activation of apoptotic and cell cycle arrest-associated genes upon nSREBP2 induction. A: RNA-seq analysis of dox-induced HeLa-pTet-nSREBP2 cells. Cells were treated with 0 or 0.3 μg/ml dox for 8 h. B: KEGG results for upregulated pathways using dox inducible genes from Α. C: Cell cycle pathway analysis from B. D: QPCR analysis of cell cycle-related genes. Data are represented as mean ± SD. E: Western blotting analysis was used to detect the expression of p21 and p57 with or without the induction of nSREBP2. HeLa and HeLa-pTet-nSREBP2 cells were treated with 0/0.3 μg/ml dox for 16 h. F: Heatmap of representative apoptosis-related genes from Β. G: QPCR analysis of apoptosis-related genes. Data are represented as mean ± SD. H: Immunoblot of cytochrome C, Tom20, and GAPDH in HeLa-pTet-nSREBP2 cells. Cells were treated with or without 0.3 μg/ml dox for 12 h and 16 h. Mitochondria and cytosol fractions were separated for immunoblotting.