Fig. 5.

nSREBP2 induced lytic cell death is independent of gasdermins or MLKL. A: cell viability of WT and ABCDE-5KO HeLa-pTet-nSREBP2 cells were analyzed by cell counting kit-8. WT and ABCDE-5KO HeLa-pTet-nSREBP2 cells were treated with or without 0.3 μg/ml dox for 24 h. Data are presented as means ± SD. Statistical analyses, unpaired two-tailed Student’s t test. B: Crystal violet staining of the effects of MLKL-KO in HeLa-pTet-nSREBP2 cells. C: Dox-induced expression of nSREBP2 caused LDH release in both WT and MLKL-KO cells. D: LDH release test. E: representative confocal images of WT, ABCDE-5KO and MLKL-KO, and ABCDEM-6KO HeLa-pTet-nSREBP2 cells. All cells were induced by 0.3 μg/ml dox for 24 h, then imaged at 40× magnification at 5 min intervals for 12 h. Scale bar, 25 μm. Real-time videos are included in supplemental Video S5. ns, not significant.