Figure 2. ADT stimulates the PV accumulation of PD-1-CD8+T cells in mouse (Myc-CaP) (A, B) and human (C, D) prostate tumors. (A, B). (A) Representative fluorescence images showing the presence of mainly PD-1- CD8+T cells in PV areas of ADT-treated Myc-CaP (A) and human (C) prostate tumors (left panels in both). (A, C) Yellow arrows=PD-1+CD8+T cells, orange arrows=PD-1-CD8+T cells. ADT stimulates the PV accumulation of CD8+T cells (A, C, right panels), which are mainly PD-1- (B, D left panels). The majority of CD8+T cells lack expression of PD-1 across tumors, which increases further after ADT (B, D, right panels). (NPV=non-PV). Data are presented as means±SEMs. Fluorescence images in A, C are from ADT-treated tumors. *p<0.05, **p<0.01, ***p<0.001. Magnification bars=20 µm. ADT, androgen deprivation therapy; PV, perivascular.