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. 2024 Jul 27;12(7):e009232. doi: 10.1136/jitc-2024-009232

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© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Characteristics of TLSs maturation heterogeneity in BCBMs. IHC assay to evaluate aggregates of lymphocytes that have histological features analogous to lymphoid tissues with CD3⁺ T cells, CD20⁺ follicular B cells, CD21⁺ follicular DCs, or CD23⁺ germinal center cells in serial sections. (A) Representative images of early TLSs. (B) Representative images of primary follicle-like TLSs. BCBM, breast cancer brain metastases; CD, cluster of differentiation; DC, dendritic cells; IHC, immunohistochemistry; TLS, tertiary lymphoid structure.