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. 2024 Jul 27;12(7):e009232. doi: 10.1136/jitc-2024-009232

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© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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The heterogeneous distribution of immune cells within TLSs. (A) Representative multiplex immunofluorescence images showing the positivity of CD3, CD8, CD20, CD68, CD163, Foxp3, PD-1, PD-L1, TIM-3, and CK in intratumoral TLSs. (B) Comparison of the percentage of specific cell types among DAPI-positive cells using one-way ANOVA. ANOVA, analysis of variance; CD, cluster of differentiation; CK, cytokeratin; DAPI, 4′,6-diamidino-2-phenylindole; PD-1, programmed cell death protein 1; PD-L1, programmed death-ligand 1; TIM-3, T cell immunoglobulin domain and mucin domain-3; TLS, tertiary lymphoid structure.