FIG. 1.
Strategy for generation of the HCMV BAC plasmid. (A) The structure of the HCMV AD169 genome with the UL and US components is shown at the top. The terminal and internal repeat sequences are indicated as open boxes. (B) The BAC vector and the selection marker gpt were integrated between the US1 and US7 genes in the US region of the viral genome by homologous recombination in fibroblasts with the recombination plasmid pEB1097. (C) Genomic structure of the resulting reconstituted virus RVHB5 and the corresponding BAC plasmid pHB5. The sizes of expected HindIII (H) and EcoRI fragments (E) in the parental virus genome and in the RVHB5 genome and BAC plasmid pHB5 are indicated. b′, a′, and c′ mark components of the internal repeat sequences. The illustration is not drawn to scale.