FIG. 4.
Multiple-step growth curve analysis of the reconstituted virus RVHB5 and of the parental HCMV strain AD169. HFF cells seeded in six-well dishes (5 × 105 cells/well) were infected with an MOI of 0.1. At the indicated time points (days postinfection) supernatants from the infected cultures were harvested, and total PFU of infectious virus in the culture supernatants were determined by plaque assay on HFF cells. Each data point represents the average of three independent wells. Day 0 titers represent input inocula.