Figure 4. Curcumin inhibited renal infiltration and the production of inflammatory cytokines by neutrophils in LN. (A) Immunohistochemical staining of Ly6G in the kidneys of MRL/lpr mice treated with or without curcumin (scale bar: 50 μm). (B) Immunohistochemical staining of Ly6G in kidneys from C57BL/6J mice in the control, R848 and R848+curcumin treatment groups (scale bar: 50 μm). (C) Flow cytometric analysis of neutrophils in the kidney and peripheral blood of MRL/lpr mice treated with or without curcumin (n=3). (D) RT-qPCR detection of the mRNA expression of Tnfα, Ifnγ, Il-1β, Il-6, Mrp8 and Mrp14 in the neutrophils of blood from MRL/lpr mice treated with or without curcumin (n=6). (E) RT-qPCR detection of the mRNA expression of Tnfα, Ifnγ, Il-1β, Il-6, Mrp8 and Mrp14 in neutrophils in blood from C57BL/6J mice in the control, R848 and R848+curcumin treatment groups (n=5). (F) RT-qPCR detection of the mRNA expression of TNFα, IFNα, IFNβ, IFNγ, IL-1β, IL-6 and IL-8 in neutrophils from the blood of patients with LN (n=5). The data are presented as the mean±SEM. *P<0.05, **p<0.01, ***p<0.001. IFN, interferon; IL, interleukin; mRNA, messenger RNA; TNF, tumour necrosis factor.