Figure 3. Immunotypes and secretomes across 11 HPV-negative head and neck squamous cell carcinoma resection specimens. (A) Hierarchical clustering of normalized protein expression (NPX) levels of 64 proteins (x-axis) measured in overnight supernatants of matched fresh single-cell suspensions from 11 tumors (y-axis). (B) Secretome comparison between fully infiltrated tumors versus other immunotypes. Protein levels (in normalized protein expression (NPX) values) measured in overnight cultures of 100,000 single cells from matched tumors on y-axis, in fully infiltrated tumors (n=5) and tumors with other immunotypes (immune-desert n=1, immune-excluded n=2 and stroma-restricted n=3) on x-axis, p values obtained by unpaired non-parametric Mann-Whitney tests, bars represent median. CCL, CC motif chemokine ligand; CXCL, C-X-C motif chemokine ligand; IFN, interferon; IL, interleukin.