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. 2024 Jul 29;55:94. doi: 10.1186/s13567-024-01350-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Analysis of brain, spinal cord and lymph node homogenates by protein misfolding cyclic amplification (PMCA). Western blots of the 4th-6th PMCA rounds are shown. After 6 rounds of amplification, PrP^Sc was detected with the monoclonal antibody 6D11 in 5 out of 65 samples acquired from the inoculated sheep. Thirty-eight of these samples are shown here. Samples from healthy controls and from sheep subjected to classical scrapie were used as controls. All amplified PrP^Sc were characterized by a predominant di-glycosylated band. The numbers on the right of each WB indicate the molecular weight marker in kilodaltons (kDa). The amplified sample from the thoracic spinal cord of sheep 90506 is not shown but can be seen in Additional file 6, along with the remaining wells that are not included in this figure. Ln: lymph node. PK: proteinase K. C. Scrapie: classical scrapie.