FIG. 5.
Effect of D609 on RSV growth and P protein phosphorylation. The various concentrations of D609 used in vitro and ex vivo are indicated. The effect of D609 on CK2 activity in vitro (○) was tested by using recombinant RSV P as a substrate and total HEp-2 extract as the source of CK2 in a standard kinase reaction; use of purified CK2 produced essentially similar results. Ex vivo effects of D609 were determined by monitoring progeny viral titer (▵) and metabolic labeling of RSV P protein (●) by 32Pi. All data are presented as percentages of values obtained with samples not treated with D609.