TABLE 2.

Relative change in reporter gene expression caused by FMDV L protease^a

Construct	Relative change (%) in expression of:
	Neomycin phosphotransferase	β-Gal
pEMCV-CBD 260–837	−85.5	+145
pVL30m 362–1144	−35.5	+281.5
pVL30m 362–575	−29	+359
pVL30m 362–461	−71	+102.5
pVL30m 462–1144	−59.5	+302.5
pVL30m 576–1144	−63.5	+154.5

^aThe gel shown in Fig. 3 was scanned, and data were used to calculate the effect of L protease on the percentage increase or decrease relative to translation in untreated Flexi-RRL preparations [100% − (level of reporter gene in the presence of L protease) × 100%/(level of reporter gene in the absence of L protease)] (54).