TABLE 3.
Recombinant bicistronic retroviral titera
| Vector | Retroviral titer (TU/ml) |
|---|---|
| pVL30m-SU 8 | 2 |
| pVL30m-SU 9 | 1.4 × 103 |
| pVL30m-SU 11 | 2.0 × 107 |
| pVL30m-SU 12 | 1.0 × 105 |
| pRev-HW 3 | 9 × 108 |
| pEMCV-CBT4 | 3.3 × 108 |
a
Viral titers were determined by performing serial dilutions followed by PLAP histochemical staining (54, 83). NIH 3T3 cells were seeded 24 h prior to transduction with virus-containing medium harvested from stably transformed ecotropic GP+E-86 cells (56). One-milliliter volumes of virus-containing medium were directly used to transduce 8 × 105 NIH 3T3 cells, PLAP-positive colonies were counted after 15 days of G418 selection. The viral titers are reported in PLAP-positive TU per milliliter of collected medium and correspond to the means of triplicate experiments. standard deviations did not exceed 15% of mean values.