Table 1.
Kinetic binding affinities of monomeric nanobodies for the RBDs of SARS-CoV-2 measured by biolayer interferometry [25].
| nanobody | antigen | average K D (nM) | average k a (1/Ms) | average k dis (1/s) |
|---|---|---|---|---|
| A8 monomer | Beta RBD | 0.889 | 3.26E+05 | 2.89E−04 |
| BA.1 RBD | 15.7 | 3.43E+05 | 5.30E−03 | |
| BA.4/5 RBD | 8.10 | 3.49E+05 | 2.83E−03 | |
| H6 monomer | Beta RBD | 0.677 | 4.98E+05 | 3.37E−04 |
| BA.1 RBD | 22.7 | 2.45E+05 | 5.53E−03 | |
| BA.4/5 RBD | 7.08 | 3.33E+05 | 2.35E−03 | |
| B5-5 monomer | Beta RBD | 0.0655 | 2.16E+05 | 1.29E−05 |
| BA.1 RBD | 0.355 | 5.76E+05 | 2.04E−04 | |
| BA.4/5 RBD | 0.684 | 1.90E+05 | 1.29E−04 | |
| A10-5 monomer | Beta RBD | <1.0 pM | 1.78E+05 | <1.0E−07 |
| BA.1 RBD | <1.0 pM | 7.55E+04 | <1.0E−07 | |
| BA.4/5 RBD | <1.0 pM | 8.09E+04 | <1.0E−07 | |
| A3-8 monomer | BA.1 RBD | 0.0258 | 3.52E+05 | 9.09E−06 |
| BA.4/5 RBD | 424 | 4.22E+05 | 0.179 | |
| A4-8 monomer | BA.1 RBD | 1.25 | 5.68E+05 | 2.03E−04 |
| BA.4/5 RBD | 6.17 | 2.03E+05 | 1.25E−03 |