Figure 2.

Multiple-sequence alignment of INS superfamily members. Highlighted in blue are strictly conserved catalytic residues (*), in red are highly similar residues (:), and in purple are moderately similar residues (.). The MCP3 sequence highlighted in orange is the unique N-terminal domain. Residue conservation determined and scored with the BLOSUM62 matrix via the MAFFT7 alignment tool. Clustal Omega was used to generate the alignment figure. Tb ProRS sequence represents the N-terminal ProXp-ala domain (94–260). The alignment presented represents the regions with the highest similarity—the entire sequence of each protein is not shown. Tb, Trypanosoma brucei; At, Arabidopsis thaliana; Cc, Caulobacter crescentus; Rp, Rhodopseudomonas paulustris; Ec, Escherichia coli; Hs, Homo sapiens.